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Overview

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  IP antibody use：5ug His Mouse IgG1 per ml Lysate，WB 1:3000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with His-tag fusion protein 3、IP (untransfected 293+anti-His mAb+ Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Prote
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  IF analysis of 293 cells transfected with a His-tag protein, using ELKbio anti-HisTag Mouse mAb at a 1:1000 dilution (blue DAPI, red anti-His)
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  2ug His fusion protein+ Primary antibody dilution at 1、1:5,000 2、1:10,000
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  1、Multi-Tag Recombinant protein 2、293T transfected with His recombinant protein 3、Insect cell trandfected with His recombinant protein