8-羥基脫氧鳥苷(8-OHdG)酶聯免疫吸附檢測試劑盒
一鍵復制產品信息 規格： |
 價格： |
|---|---|
| 48T | ￥1680.00 |
| 96T | ￥2400.00 |
| 96T*5 | ￥10200.00 |
下載說明 ①Overview 文獻(5)
日期：2022-03-08
IF：5.3標準曲線
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 100.00 | 0.221 | |
| 50.00 | 0.411 | |
| 25.00 | 0.692 | |
| 12.50 | 0.913 | |
| 6.25 | 1.225 | |
| 3.13 | 1.587 | |
| 1.57 | 1.813 | |
| 0.00 | 2.325 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant 8-OHdG and the recovery rates were calculated by comparing the measured value to the expected amount of 8-OHdG in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 94-107% | 100% |
| EDTA plasma(n=5) | 82-95% | 88% |
| Heparin plasma(n=5) | 94-105% | 87% |
線性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-OHdG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 92-105% | 92-101% | 80-93% | 89-97% |
| EDTA plasma(n=5) | 85-90% | 83-96% | 95-102% | 88-95% |
| Heparin plasma(n=5) | 92-102% | 83-89% | 88-104% | 97-103% |
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