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大鼠4-羥基壬烯酸(HNE) 酶聯免疫吸附檢測試劑盒

一鍵復制產品信息

ELK0115

規格：	價格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下載說明 ①

Overview 文獻(1)

Product name:	Rat HNE(4-Hydroxynonenal) ELISA Kit
Reactivity:	Rat
Alternative Names:	4-HNE; HNE; 4-hydroxy-2-nonenal; 4-Hydroxynonenal
Assay Type:	Sandwich
Sensitivity:	0.67 ng/mL
Standard:	100 ng/mL
Detection Range:	1.57-100 ng/mL
Sample Type:	Serum, plasma, tissue homogenates
Assay Length:	3.5h
Research Area:	Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HNE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HNE in the samples is then determined by comparing the OD of the samples to the standard curve.

NEUROSCIENCE

Short-Chain Fatty Acids on Cognitive Behaviours and Associated Neurochemistry Changes in Lps-Challenged Rats

日期：2025-05-13 IF：2.9

標準曲線

Concentration (ng/mL)	OD	Corrected OD
100.00	2.345	2.249
50.00	1.761	1.665
25.00	1.183	1.087
12.50	0.647	0.551
6.25	0.521	0.425
3.13	0.393	0.297
1.57	0.258	0.162
0.00	0.096	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant HNE and the recovery rates were calculated by comparing the measured value to the expected amount of HNE in samples.

Matrix	Recovery range	Average
serum(n=5)	78-90%	84%
EDTA plasma(n=5)	84-97%	91%
Heparin plasma(n=5)	89-105%	97%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HNE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.